<i>ACOT7</i> is a direct target of miRNA-200a.

<p>(A) The nucleotide sequence of the putative miR-200a binding site within the 3’UTR of <i>ACOT7</i>. As a negative control, a mutated version of the binding site (‘ACOT7-<i>mut</i>’) contains three different nucleotides (underlined). (B) Y79 retinoblastoma cells, transfected with a miR-200a mimic or a negative (control miR) mimic, were co-transfected with plasmid vectors containing a firefly luciferase gene coding region upstream of either the ACOT7-3’UTR (pMir-ACOT7) or the mutated ACOT7- 3’UTR (pMir-mut), in addition to a renilla luciferase plasmid for normalization purposes. Data represents mean and standard deviation from independent triplicates from two experiments. *** denotes p< 0.0001.