miR-7a/b targets poly(ADP-ribose) polymerase (PARP).

<p>(A,B) qRT-PCR of the efficiency of transfection of miR-7a/b mimic or inhibitor. N = 9, *P<0.01 vs. the control. (C) Conserved miR-7a/b binding site in 3′ untranslated region (UTR) of PARP. WT: wild type; MU: mutant type. (D, E) After miR-7a/b mimic or negative control miRNA was transfected into HEK293 cells, luciferase reporters were cotransfected into HEK293 cells for 48 h. Luciferase activity was measured and was normalized to that of Renilla. N = 6, *P<0.01, #P>0.05 vs. control. (F, G) Western blot analysis of protein level of PARP and caspase-3 with miR-7a/b mimic or inhibitor transfected into H9c2 cells. N = 3, *P<0.01 vs. control. NS, not significant.