Meningococcal cultivation procedures.

<p>A. The ideal growth times on agar plates under production of Seed Lots were 10–12 hours for the 1st passage, 7–8 hours for the 2nd passage, and 6–8 hours for the 3rd passage at 35°C. When cultivated in 1.5L medium in 2.8 L shaking flasks, shaking speed 200 rpm and temperature 35°C and an initial OD₆₅₀ = 0.1, the length of the lag phase was approximately 2 hours, whereas the log-phase seemed to end at 7–8 hours with an OD₆₅₀ of ~2. B. Comparison of growth in 1.0 L modified Frantz liquid medium in a 2L baffled shaking flask for the strains 3208 (MenPF-1 vaccine strain) and the 2851 strain (wild type H44/76 strain).