<p>(A) <i>N. meningitidis</i> strains MC58, MC58-278, MC58-1220, and MC58-278-1220 bacteria were grown in Muller-Hinton broth supplemented with 0.25% (w/v) glucose. Growth was followed over an 8-h period and the OD<sub>620</sub> was measured throughout. (B) Outer membranes were prepared from the parental strain and mutants following growth on BHI plates overnight, and separated by T-SDS-PAGE. Lane order: 1, strain MC58; 2, strain MC58-278; 3, strain MC58-1220; 4, strain MC58-278-1220; 5, whole cell lysate strain MC58.</p
<p>The ΔBPSS1356 mutant showed a reduced growth compared to the wild type after 8 hours of incubatio...
<p>Colonies of the wild type <i>T</i>. <i>marneffei</i> control strain G681, Δ<i>pbrB</i> 1&2 strain...
<p>Wild-type (<b>A</b>), and three different <i>P</i>. <i>aeruginosa nrdJ</i> insertion mutants (<b>...
<p>(A and B) Bacteria were grown in BHI at 27°C (A) and 37°C (B): parental strains WA-314 (black dia...
<p>Bacterial strains (8047, MC58 and mutants thereof) were grown to mid-log (OD600 = ~ 0.6) in MHB s...
<p>100 µl of <i>N. meningitidis</i> strain FAM20 suspended in cell culture medium DMEM was mixed wit...
<p>(A) Cell growth of wild type, <i>cdc48–353</i> and <i>cdc48–353-flag</i> strains on YES media was...
<p>A. Immunoblot of cell lysates from <i>Neisseria meningitidis</i> (Nm) Mc58 wildtype and a Mc58Δ<i...
The meningococcus has been considered one of the more fastidi-ous microorganisms with respect to gro...
<p>Wild type and Δ<i>degP</i> cells, with or without the suppressor mutation, were grown overnight a...
<p>Infections were carried out over a period of 12 h and fixed for Giemsa staining (a), (c), (d), or...
<p>The rates and extent of bacterial growth (A: EGD-e; C: Δ<i>lmo0641</i>; E:Δ<i>lmo0641</i>/pPL2<i>...
<p>The initial concentration of bacteria was 10<sup>6</sup> CFU/ml. Number of bacteria was estimated...
<p>Macrophages were seeded on 6-well bacteriological plastic plates at 1×10<sup>6</sup> cells/well a...
LB medium (A) was inoculated with an initial optical density at 600 nm of 0.04 of strains P4 strain ...
<p>The ΔBPSS1356 mutant showed a reduced growth compared to the wild type after 8 hours of incubatio...
<p>Colonies of the wild type <i>T</i>. <i>marneffei</i> control strain G681, Δ<i>pbrB</i> 1&2 strain...
<p>Wild-type (<b>A</b>), and three different <i>P</i>. <i>aeruginosa nrdJ</i> insertion mutants (<b>...
<p>(A and B) Bacteria were grown in BHI at 27°C (A) and 37°C (B): parental strains WA-314 (black dia...
<p>Bacterial strains (8047, MC58 and mutants thereof) were grown to mid-log (OD600 = ~ 0.6) in MHB s...
<p>100 µl of <i>N. meningitidis</i> strain FAM20 suspended in cell culture medium DMEM was mixed wit...
<p>(A) Cell growth of wild type, <i>cdc48–353</i> and <i>cdc48–353-flag</i> strains on YES media was...
<p>A. Immunoblot of cell lysates from <i>Neisseria meningitidis</i> (Nm) Mc58 wildtype and a Mc58Δ<i...
The meningococcus has been considered one of the more fastidi-ous microorganisms with respect to gro...
<p>Wild type and Δ<i>degP</i> cells, with or without the suppressor mutation, were grown overnight a...
<p>Infections were carried out over a period of 12 h and fixed for Giemsa staining (a), (c), (d), or...
<p>The rates and extent of bacterial growth (A: EGD-e; C: Δ<i>lmo0641</i>; E:Δ<i>lmo0641</i>/pPL2<i>...
<p>The initial concentration of bacteria was 10<sup>6</sup> CFU/ml. Number of bacteria was estimated...
<p>Macrophages were seeded on 6-well bacteriological plastic plates at 1×10<sup>6</sup> cells/well a...
LB medium (A) was inoculated with an initial optical density at 600 nm of 0.04 of strains P4 strain ...
<p>The ΔBPSS1356 mutant showed a reduced growth compared to the wild type after 8 hours of incubatio...
<p>Colonies of the wild type <i>T</i>. <i>marneffei</i> control strain G681, Δ<i>pbrB</i> 1&2 strain...
<p>Wild-type (<b>A</b>), and three different <i>P</i>. <i>aeruginosa nrdJ</i> insertion mutants (<b>...