Loss of <i>flp</i> genes results in defects in locomotion.

<p>(A) Disruption of multiple <i>flp</i> genes affects swimming rate. One day hermaphrodite adults were scored for the number of body bends per 15 seconds in physiological M9 buffer. (B) Many <i>flp</i> mutants show resistance to serotonin-induced inhibition on swimming locomotion. One day hermaphrodite adults were scored for the number of body bends per 15 seconds in physiological M9 buffer with 12.9 mM serotonin. Mean and SEM shown. N = number of animals examined; each animal was tested three times and the values averaged for that animal. At least three trials were performed for each strain. Mutants are color-coded and were grouped according to predominant expression pattern; loco. IN, <i>flp</i> expression in locomotory interneurons; SN, <i>flp</i> expression predominantly in sensory neurons; MN, <i>flp</i> expression predominantly in motoneurons; IN, <i>flp</i> expression predominantly in interneurons; solid bars indicate mutants, patterned bars indicate mutants containing corresponding genomic fragment; transgenic animals also indicated by [], Ex indicates non-integrated array, Is indicates integrated array; <i>myo-2</i>p::<i>GFP</i> and/or <i>sur-5</i>::<i>GFP</i> were used as the transgenic marker (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0135164#pone.0135164.t001" target="_blank">Table 1</a>). *, p<0.05, **, p<0.01, ***, p<0.001 significantly different from wild type, Mann-Whitney test; ^, p<0.05, ^^, p<0.01, ^^^, p<0.001 significantly different from mutant, one-way ANOVA, Neuman Keuls posthoc test.