HPV induces overexpression of SIRT1 throughout keratinocyte differentiation.

<p><b>(A)</b> Western blot analysis of SIRT1 in normal HFKs. HFKs were stably transfected with HPV 31 or 16 genomes, and CIN612 cells. <b>(B)</b> Western blot analysis of other SIRT family members in the same cells as in (A) <b>(C)</b> Western blot analysis of HFKs and CIN612 cells induced to differentiate using 1.5mM calcium chloride for up to 96 hours. Cytokeratin 10 (K10) is a marker of differentiation. <b>(D)</b> Protein blot signal intensity analysis was performed using Licor Image Studio software on results from at least three independent experiments. Error bars represent +/- 1 SEM. Significance as determined by Student’s t-test is shown as * = p<0.05, ** = p<0.005.