SIRT1 binds the HPV31 URR and controls histone acetylation.

<p><b>(A)</b> CIN612 cells were grown undifferentiated in monolayer culture or induced to differentiate in high-calcium media for 72 hours and ChIP-pPCR was performed for the HPV URR. Results shown are the average Ct IP/Ct IgG using isotype matched IgG control antibody. Ct values were normalized to HPV genome input copy number. Error bars are +/- SEM of the average of three PCR replicates between three independent experiments. Significance of differences as determined by Student’s t-test is shown as * = p<0.05 <b>(B)</b> Histones were acid extracted and electrophoresed on SDS-PAGE gel. Total histone 3 (H3) serves as a loading control. <b>(C)</b> Monolayer CIN612 cells were transduced for 72 hours and ChIP-qPCR for the HPV31 URR performed as in (A). Results shown are the average fold enrichment relative to isotype matched IgG control antibody. Ct values were normalized to HPV genome input copy number. Error bars are +/- SEM of the average of three PCR replicates between three independent experiments.