Sensitivity of the <i>L</i>. <i>loa</i> RF4-based LAMP assay.

<p>Dilutions of genomic <i>L</i>. <i>loa</i> DNA were amplified with the RF4 primer set and <i>Bst</i> 2.0 DNA polymerase in the absence (blue) or presence (red) of the V/DEF additive. Two ul of each dilution was added to LAMP reactions. The average threshold time, defined as the time at which the change in turbidity over time (dT/dt) reaches a value of 0.1, is plotted against the concentration of <i>L</i>. <i>loa</i> DNA (ng/ml). All reactions were performed in triplicate. Error bars represent the standard deviation at each point.