The real-time amplification of the STY2879 gene by LAMP.

<p>A. The real-time sensitivity of STY2879-LAMP as monitored by measurement of turbidity (optimal density at 650 nm). A turbidity of ≥ 0.1 was considered to be positive for STY2879-LAMP. Ten-fold serial dilutions of the reference plasmid pEASY-T1-STY2879 ranging from 1.5 × 10⁵ to 10⁰ copies/reaction were tested. B. The relationship between the threshold time (Tt) of each sample and the log copies/reaction. The standard curve was drawn on the basis of three independent repeats and the linear relationship R² = 0.9804. C. A direct visual detection by the naked eye with the addition of the FDR dye, which was added prior to the amplification. The original orange color of the FDR changed to green in the case of a positive amplification under natural light, whereas the original orange color was retained for a negative reaction.