qRT-PCR analysis of the relative gene expression level of NF3-treated HUVEC versus control HUVEC at 6 h.

qRT-PCR validation of three differentially expressed genes from thrombin-treated HMVEC RNA-Seq data. qRT-PCR was carried out as described in the Method.

Li Qin Zhang (183207)
Dilyara Cheranova (183213)
Margaret Gibson (183218)
Shinghua Ding (183224)
Daniel P. Heruth (183227)
Deyu Fang (183231)
Shui Qing Ye (3623)

February 2012

Fold changes determined from the relative Ct values of TaqMan Gene Expression assay for CUGBP, El...

Validation via qRT-PCR of four genes found to be significant in the array analysis.

Franny Jongbloed (587952)
Ron W. F. de Bruin (519863)
Jeroen L. A. Pennings (224558)
César Payán-Gómez (587953)
Sandra van den Engel (372996)
Conny T. van Oostrom (563286)
Alain de Bruin (103917)
Jan H. J. Hoeijmakers (172432)
Harry van Steeg (31742)
Jan N. M. IJzermans (283157)
Martijn E. T. Dollé (34343)

June 2014

Fold ratios and corresponding p-values obtained via microarray analysis (Affy) and qRT-PCR (qPCR)...

Detection of miRNAs of interest by qRT-PCR in various cell lines.

Alexandre Marchand (309383)
Carole Proust (108244)
Pierre-Emmanuel Morange (161602)
Anne-Marie Lompré (309385)
David-Alexandre Trégouët (133741)

February 2013

(1)MiRNAs with Cycle Threshold (Ct) values over 31 were considered not sufficiently expressed for...

qRT-PCR analysis of the relative gene expression level of NF3-treated HUVEC versus control HUVEC at 16 h.

Jacqueline Chor Wing Tam (802881)
Chun Hay Ko (802882)
Chi Man Koon (802883)
Zhang Cheng (802884)
Wong Hing Lok (802885)
Ching Po Lau (802886)
Ping Chung Leung (420415)
Kwok Pui Fung (802887)
Wai Yee Chan (503798)
Clara Bik San Lau (623380)

October 2015

Values are means ± SD from three individual experiments.RNA from new preparation of HUVEC ...

Validation of microarray data with qRT-PCR.

Flavia S. Lakschevitz (432780)
Guy M. Aboodi (432781)
Michael Glogauer (71333)

July 2013

(A) Quantitative real time PCR was used to quantify gene expression of selected genes from neutro...

Validation of microarray results of selected genes in H4 cells by qRT-PCR.

Shuangshuang Guo (620194)
Yuming Guo (162863)
Ayla Ergun (728901)
Lei Lu (318582)
W. Allan Walker (354413)
Kriston Ganguli (728902)

April 2015

The RNA used for qRT-PCR was from the same samples used for microarray analysis. NF-κB activation...

U-II activated ATF3 in HUVECs. HUVECs were treated with 10 nM U-II for the indicated time, and mRNA levels of ATF3 were evaluated by PCR.

Yen-Ling Chen (636565)
Yi-Ting Tsai (309320)
Chung-Yi Lee (516125)
Chien-Hsing Lee (312747)
Chung-Yi Chen (636566)
Chi-Ming Liu (636567)
Jin-Jer Chen (525834)
Shih-Hurng Loh (525831)
Chien-Sung Tsai (484479)

September 2014

(A) Equal RNA loadings were documented by GAPDH (lower). (B) HUVECs were treated with 10 nM U-II ...

Validation of expression levels of genes in microarray by qRT-PCR.

Nicholas Hatzirodos (280504)
Helen F. Irving-Rodgers (280503)
Katja Hummitzsch (280502)
Raymond J. Rodgers (280509)

June 2014

qRT-PCR gene expression values were determined from the mean of the ratio of the ΔCt for the targ...

Validation of the expression of selected transcripts by qRT-PCR.

Anne Jaerve (112641)
Fabian Kruse (112644)
Katharina Malik (112646)
Hans-Peter Hartung (112650)
Hans Werner Müller (112654)

December 2012

Validation of changes in mRNA expression by quantitative real-time RT-PCR (qRT-PCR) for genes rep...

Validation of gene array data by qRT-PCR.

Vasu Punj (15504)
Hittu Matta (71253)
Preet M. Chaudhary (163585)

May 2012

(A) Twenty five genes from NF-κB, cytokine, and inflammatory pathways were randomly selected and ...

Scatter plot illustrating correlations of array results to qRT-PCR validation.

Christopher K. Thompson (170958)
John Meitzen (170962)
Kirstin Replogle (46753)
Jenny Drnevich (170966)
Karin L. Lent (170969)
Anne Marie Wissman (170971)
Federico M. Farin (128418)
Theo K. Bammler (128417)
Richard P. Beyer (128416)
David F. Clayton (170979)
David J. Perkel (170983)
Eliot A. Brenowitz (170987)

April 2012

The expression of 19 genes from five time points relative to SD-long term comparing results using...

Correlation of gene expression ratios between cDNA microarray and qRT-PCR.

Chia-Yang Li (149991)
Chi-Shiun Chiang (149996)
Wei-Chung Cheng (150004)
Shu-Chi Wang (150010)
Hung-Tsu Cheng (150017)
Chaang-Ray Chen (150023)
Wun-Yi Shu (150031)
Min-Lung Tsai (150040)
Ruey-Shyang Hseu (150046)
Cheng-Wei Chang (150052)
Chao-Ying Huang (150059)
Shih-Hua Fang (150067)
Ian C. Hsu (150075)

July 2012

A total of 36 microarray samples were validated by qRT-PCR (6 representative genes in different t...

Confirmation of integrity of the microarray data qRT-PCR analysis.

Amanda Whitmill (5680232)
Seongcheol Kim (198951)
Vivian Rojas (5680235)
Fahad Gulraiz (586860)
Kazi Afreen (5680238)
Mamta Jain (623611)
Meharvan Singh (5680241)
In-Woo Park (5656204)

August 2018

RNAs isolated from the indicated stages of 3 of each mono- and co-infected patients’ liver samples w...

Validation of microarray data by qRT-PCR.

Gennifer Goode (578434)
Siddharth Pratap (316339)
Sakina E. Eltom (578435)

June 2014

Differential gene expression from the microarrays analyses was confirmed by qRT-PCR of 10 selecte...

Validation of microarray results by qRT-PCR analysis.

Matheus Correa-Costa (119464)
Hátylas Azevedo (119466)
Mariane Tami Amano (119467)
Giselle Martins Gonçalves (119469)
Meire Ioshie Hyane (119471)
Marcos Antonio Cenedeze (119473)
Paulo Guilherme Renesto (119476)
Alvaro Pacheco-Silva (119478)
Carlos Alberto Moreira-Filho (119482)
Niels Olsen Saraiva Câmara (119484)

November 2012

Expression of the genes Hmox-1 (A), Fosl1 (B) and Cxcl1 (C), were evaluated by microarray and qRT...

qRT-PCR validation of three differentially expressed genes from thrombin-treated HMVEC RNA-Seq data. qRT-PCR was carried out as described in the Method.

Li Qin Zhang (183207)
Dilyara Cheranova (183213)
Margaret Gibson (183218)
Shinghua Ding (183224)
Daniel P. Heruth (183227)
Deyu Fang (183231)
Shui Qing Ye (3623)

February 2012

Fold changes determined from the relative Ct values of TaqMan Gene Expression assay for CUGBP, El...

Validation via qRT-PCR of four genes found to be significant in the array analysis.

Franny Jongbloed (587952)
Ron W. F. de Bruin (519863)
Jeroen L. A. Pennings (224558)
César Payán-Gómez (587953)
Sandra van den Engel (372996)
Conny T. van Oostrom (563286)
Alain de Bruin (103917)
Jan H. J. Hoeijmakers (172432)
Harry van Steeg (31742)
Jan N. M. IJzermans (283157)
Martijn E. T. Dollé (34343)

June 2014

Fold ratios and corresponding p-values obtained via microarray analysis (Affy) and qRT-PCR (qPCR)...

Detection of miRNAs of interest by qRT-PCR in various cell lines.

Alexandre Marchand (309383)
Carole Proust (108244)
Pierre-Emmanuel Morange (161602)
Anne-Marie Lompré (309385)
David-Alexandre Trégouët (133741)

February 2013

(1)MiRNAs with Cycle Threshold (Ct) values over 31 were considered not sufficiently expressed for...

qRT-PCR analysis of the relative gene expression level of NF3-treated HUVEC versus control HUVEC at 16 h.

Jacqueline Chor Wing Tam (802881)
Chun Hay Ko (802882)
Chi Man Koon (802883)
Zhang Cheng (802884)
Wong Hing Lok (802885)
Ching Po Lau (802886)
Ping Chung Leung (420415)
Kwok Pui Fung (802887)
Wai Yee Chan (503798)
Clara Bik San Lau (623380)

October 2015

Values are means ± SD from three individual experiments.RNA from new preparation of HUVEC ...

Validation of microarray data with qRT-PCR.

Flavia S. Lakschevitz (432780)
Guy M. Aboodi (432781)
Michael Glogauer (71333)

July 2013

(A) Quantitative real time PCR was used to quantify gene expression of selected genes from neutro...

Validation of microarray results of selected genes in H4 cells by qRT-PCR.

Shuangshuang Guo (620194)
Yuming Guo (162863)
Ayla Ergun (728901)
Lei Lu (318582)
W. Allan Walker (354413)
Kriston Ganguli (728902)

April 2015

The RNA used for qRT-PCR was from the same samples used for microarray analysis. NF-κB activation...

U-II activated ATF3 in HUVECs. HUVECs were treated with 10 nM U-II for the indicated time, and mRNA levels of ATF3 were evaluated by PCR.

Yen-Ling Chen (636565)
Yi-Ting Tsai (309320)
Chung-Yi Lee (516125)
Chien-Hsing Lee (312747)
Chung-Yi Chen (636566)
Chi-Ming Liu (636567)
Jin-Jer Chen (525834)
Shih-Hurng Loh (525831)
Chien-Sung Tsai (484479)

September 2014

(A) Equal RNA loadings were documented by GAPDH (lower). (B) HUVECs were treated with 10 nM U-II ...

Validation of expression levels of genes in microarray by qRT-PCR.

Nicholas Hatzirodos (280504)
Helen F. Irving-Rodgers (280503)
Katja Hummitzsch (280502)
Raymond J. Rodgers (280509)

June 2014

qRT-PCR gene expression values were determined from the mean of the ratio of the ΔCt for the targ...

Validation of the expression of selected transcripts by qRT-PCR.

Anne Jaerve (112641)
Fabian Kruse (112644)
Katharina Malik (112646)
Hans-Peter Hartung (112650)
Hans Werner Müller (112654)

December 2012

Validation of changes in mRNA expression by quantitative real-time RT-PCR (qRT-PCR) for genes rep...

Validation of gene array data by qRT-PCR.

Vasu Punj (15504)
Hittu Matta (71253)
Preet M. Chaudhary (163585)

May 2012

(A) Twenty five genes from NF-κB, cytokine, and inflammatory pathways were randomly selected and ...

Scatter plot illustrating correlations of array results to qRT-PCR validation.

Christopher K. Thompson (170958)
John Meitzen (170962)
Kirstin Replogle (46753)
Jenny Drnevich (170966)
Karin L. Lent (170969)
Anne Marie Wissman (170971)
Federico M. Farin (128418)
Theo K. Bammler (128417)
Richard P. Beyer (128416)
David F. Clayton (170979)
David J. Perkel (170983)
Eliot A. Brenowitz (170987)

April 2012

The expression of 19 genes from five time points relative to SD-long term comparing results using...

Correlation of gene expression ratios between cDNA microarray and qRT-PCR.

Chia-Yang Li (149991)
Chi-Shiun Chiang (149996)
Wei-Chung Cheng (150004)
Shu-Chi Wang (150010)
Hung-Tsu Cheng (150017)
Chaang-Ray Chen (150023)
Wun-Yi Shu (150031)
Min-Lung Tsai (150040)
Ruey-Shyang Hseu (150046)
Cheng-Wei Chang (150052)
Chao-Ying Huang (150059)
Shih-Hua Fang (150067)
Ian C. Hsu (150075)

July 2012

A total of 36 microarray samples were validated by qRT-PCR (6 representative genes in different t...

Confirmation of integrity of the microarray data qRT-PCR analysis.

Amanda Whitmill (5680232)
Seongcheol Kim (198951)
Vivian Rojas (5680235)
Fahad Gulraiz (586860)
Kazi Afreen (5680238)
Mamta Jain (623611)
Meharvan Singh (5680241)
In-Woo Park (5656204)

August 2018

RNAs isolated from the indicated stages of 3 of each mono- and co-infected patients’ liver samples w...

Validation of microarray data by qRT-PCR.

Gennifer Goode (578434)
Siddharth Pratap (316339)
Sakina E. Eltom (578435)

June 2014

Differential gene expression from the microarrays analyses was confirmed by qRT-PCR of 10 selecte...

Validation of microarray results by qRT-PCR analysis.

Matheus Correa-Costa (119464)
Hátylas Azevedo (119466)
Mariane Tami Amano (119467)
Giselle Martins Gonçalves (119469)
Meire Ioshie Hyane (119471)
Marcos Antonio Cenedeze (119473)
Paulo Guilherme Renesto (119476)
Alvaro Pacheco-Silva (119478)
Carlos Alberto Moreira-Filho (119482)
Niels Olsen Saraiva Câmara (119484)

November 2012

Expression of the genes Hmox-1 (A), Fosl1 (B) and Cxcl1 (C), were evaluated by microarray and qRT...

qRT-PCR validation of three differentially expressed genes from thrombin-treated HMVEC RNA-Seq data. qRT-PCR was carried out as described in the Method.

Li Qin Zhang (183207)
Dilyara Cheranova (183213)
Margaret Gibson (183218)
Shinghua Ding (183224)
Daniel P. Heruth (183227)
Deyu Fang (183231)
Shui Qing Ye (3623)

February 2012

Fold changes determined from the relative Ct values of TaqMan Gene Expression assay for CUGBP, El...

Validation via qRT-PCR of four genes found to be significant in the array analysis.

Franny Jongbloed (587952)
Ron W. F. de Bruin (519863)
Jeroen L. A. Pennings (224558)
César Payán-Gómez (587953)
Sandra van den Engel (372996)
Conny T. van Oostrom (563286)
Alain de Bruin (103917)
Jan H. J. Hoeijmakers (172432)
Harry van Steeg (31742)
Jan N. M. IJzermans (283157)
Martijn E. T. Dollé (34343)

June 2014

Fold ratios and corresponding p-values obtained via microarray analysis (Affy) and qRT-PCR (qPCR)...

Detection of miRNAs of interest by qRT-PCR in various cell lines.

Alexandre Marchand (309383)
Carole Proust (108244)
Pierre-Emmanuel Morange (161602)
Anne-Marie Lompré (309385)
David-Alexandre Trégouët (133741)

February 2013

(1)MiRNAs with Cycle Threshold (Ct) values over 31 were considered not sufficiently expressed for...

qRT-PCR analysis of the relative gene expression level of NF3-treated HUVEC versus control HUVEC at 6 h.

Abstract

Extracted data

qRT-PCR analysis of the relative gene expression level of NF3-treated HUVEC versus control HUVEC at 6 h.

Abstract

Extracted data

Related items

Related items