Overexpression of mitochondrial MsrA does not alter mitochondrial content.

<p>(A) Representative blot of MsrA expression in muscle. (B) Representative blot of subcellular distribution of MsrA in muscle. (1,000s) = 1,000 x g supernatant, 10,000s = 10,000 x g supernatant, 10,000p = 10,000 x g pellet. (C) Citrate synthase activity in muscle from SD- and HFD-fed mice. Asterisks and horizontal line indicate significant effect of diet as measured by two way ANOVA. (D) Quantification of western blots for Complex I subunit NDUFB8 (Complex I), Complex II subunit 30kDa (Complex II), Complex III subunit Core 2 (Complex III), Complex IV subunit I (Complex IV), and ATP synthase subunit alpha (Complex V). Values are given as arbitrary units (AU) normalized among groups to levels of VDAC. Bars represent mean (relative to values in WT) ± SEM. Asterisks and horizontal line indicate significant effect of diet as measured by two way ANOVA.