HA-hMC4R and hMRAPα-FLAG co-localised together with markers of the Golgi apparatus and the ER in HEK293 cells.

<p>HA-hMC4R and hMRAPα-FLAG were transiently transfected into HEK293 cells stably expressing DsRed-ER, a marker of the endoplasmic reticulum (A, C, E, G, I, K) or GalTase-mCherry, a marker of the Golgi apparatus (B, D, F, H, J, L). Co-localisation of HA-hMC4R and DsRed-ER or GalTase-mCherry is observed as yellow spots and marked by a white arrow in panels G and H. HA-hMC4R and hMRAPα-FLAG co-localisation is observed as aqua coloured spots and marked by a white arrow in panels I and J. Co-localisation of HA-hMC4R and hMRAPα-FLAG and DsRed-ER or GalTase-mCherry is observed as whitish spots and marked by a white arrow in panels K and L. Co-localisation for the data represented by panels G and H in this figure is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140320#pone.0140320.s004" target="_blank">S2A Fig</a> Scale bars = 10μm.