hMC4R-eGFP lacked complex N-linked glycosylation when expressed in HEK293 cells.

<p>HEK293 cells were transiently co-transfected with hMC4R-eGFP and hMRAPα, hMRAPα-FLN or the empty vector pcDNA 3.1 and western blotting was performed with anti-GFP antibody (A). Lysates for the same transfections were also treated with PNGase F and endoglycosidase H to characterise hMC4R-eGFP N-linked glycosylation (B). Molecular masses (kDa) are indicated on the left side of the western blots. Bands of interest are indicated with (◀). 1–4; 1–10, lane numbers.