Immunoprecipitation analysis of the <i>in vitro</i> CotH-CotE interactions.
- Rachele Isticato (407049)
- Teja Sirec (463817)
- Stefano Vecchione (816128)
- Anna Crispino (816129)
- Anella Saggese (613957)
- Loredana Baccigalupi (435921)
- Eugenio Notomista (730473)
- Adam Driks (517296)
- Ezio Ricca (407051)
Publication date
October 2015
DOIAbstract
<p>CotH-His was bound to a Ni-NTA column and the flowthrough (FT<sub>CotH</sub>) and washes (W1-W3, here only W3 is shown) were collected. Untagged CotE was then added, and flow through (FT<sub>CotE</sub>), washed (W1—W8, here only W8 is shown), and eluted (E1—E4) proteins collected as described in Materials and Methods. Proteins were fractionated on 12.5% polyacrylamide gels, electrotransferred to membranes, and reacted with anti-CotH (A), anti-CotE (B) antibodies. The same experiment was also performed without CotH-His (C).</p
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