Effects of C-terminal deletions of CotE on <i>in vivo</i> CotH assembly.

<p>(A) The amino acid sequences of the C termini of CotE and various mutant versions. To the left of each sequence are the strain name and the number of deleted amino acids. Negatively charged amino acids when present at the C terminus are indicated in bold. Western blot with anti-CotE antibody (B), SDS-PAGE (C) and western blot with anti-CotH- antibody (D) of coat proteins extracted from spores of the strain indicated in panel A. Triangles in panel C indicate the predicted CotE bands. Proteins were fractionated on 12.5% polyacrylamide gels, electrotransferred to membranes, and reacted with anti-CotH and anti-CotE antibodies.