Assembly of CotE-dependent proteins.

<p>Proteins extracted from mature spores of a wild type strain or from isogenic strains over-producing CotH (RG24), lacking CotE (<i>cotE</i>) and over-producing CotH in the absence of CotE (RG25) were probed with anti-CotA, anti-CotB, anti-CotC and anti-CotG antibodies. Proteins were fractionated on 15% polyacrylamide gels, electrotransferred to membranes and reacted with the indicated antibodies.