FLUC expression in reporter macrophages.

<p>GFP and FLUC expressing reporter macrophages were produced by transducing mouse bone marrow macrophages with lentivirus vector in the presence of cyclosporine, DEAE-dextran or polybrene. The 24-hour virus infection was done either once or repeated after a 24-hour rest period (single vs. double infection). <b>a)</b> Reporter macrophages from the indicated treatment groups were seeded to 24-well plate at standardized density three days after the second infection. D-luciferin was added to the wells and immediately after luminescence signal emanating from each well imaged using IVIS 100 system and quantified with Living Image software. <b>b)</b> Luciferase activity in indicated double infected reporter macrophage lysates as determined by Luciferase Assay System kit and luminometer. <b>c)</b> Person correlation between the amount of GFP positive cells and normalized FLUC activity in macrophages infected once or twice in the presence of cyclosporine. D–Double infection; S–Single infection; Cy–Cyclosporine; De–DEAE-dextran; Po–Polybrene. * = p > 0.05 cyclosporine double infection vs. polybrene double infection.