Increased HIV-1 entry in LCK⁺ macrophages results in increased reverse transcription, but not successful infection.

<p>A) HIV-1 viral fusion in PSC-macrophages. The percentage of fusion of HIV-1 virions was normalised to the percentage fusion of VSV-G pseudotyped virions. Symbols represent the normalised mean fusion, relative to the PSC-macrophages control group ±SEM of independent experiments. Control-, LCK_WT P and LCK_INACTIVE P (n = 3) and control P PSC-macrophages (n = 1). B) Reverse transcription of HIV-1 in PSC-macrophages. Late (pol) products were detected by qPCR after 30 h of infection. Symbols represent the relative mean number of copies of HIV-1 DNA±SEM of independent experiments (n = 3), normalised to the number of cells using a β-actin control. C) HIV-1 productive infection in PSC-macrophages. Infection was measured by detecting luciferase activity in PSC-macrophages three days after transduction with NL4.3.Luc.R-E- virus pseudotyped with HIV-1 or VSV-G envelope. HIV-1 NL4.3.Luc.R-E- transduced cells were normalised to VSV-G NL4.3.Luc.R-E- transduced cells. Symbols represent the mean luciferase detection relative to the PSC-macrophages control group ±SEM of independent experiments (n = 3).