Reporter macrophage viability.

<p>GFP and FLUC expressing reporter macrophages were produced by transducing mouse bone marrow macrophages with lentivirus vector in the presence of cyclosporine, DEAE-dextran or polybrene. The 24-hour virus infection was done either once or repeated after a 24-hour rest period (single vs. double infection). The percentage of viable cells was assessed three days after the second infection by flow cytometry and propidium iodide staining.