Expression of ANO2 protein in Purkinje cells.

<p><b>(A)</b> ANO2 immunosignals from the cerebellar cortex are discernible in the dendrites of Purkinje cells. The signals are weak but stronger than the background signals emanating from the granule cell layer <i>(GL)</i>. <b>(B)</b> In the Purkinje cell, the ANO2 immunosignal <i>(green)</i> is visible in dendrites and the perinuclear region, but only weakly in the plasma membrane of the cell body. In contrast, ANO1 signals <i>(red)</i> label the entire Purkinje cell soma, but are not detectable in dendrites. <b>(C)</b> The ANO2 antiserum does not stain the cerebellar cortex of the Ano2^-/- mouse. <b>(D)</b> ANO2 immunosignals in the glomeruli of the olfactory bulb serving as positive control for ANO2 in brain tissue; <i>gl</i>: glomerular layer, <i>opl</i>: outer plexiform layer. <b>(E)</b> Absence of ANO2 immunosignals from the olfactory bulb of the Ano2^-/- mouse. Blue in <i>B-E</i> represents DAPI nuclear stain. All calibrations bars: 20 μm.