Cerebellar cortex.

<p>A: Arg-like immunoreactivity was observed in neurons and neuropil in all cerebellar cortical layers but was most obvious in cell bodies (white arrowheads) and processes (black arrowheads) of Bergmann glial cells. GABAergic interneurons including basket, stellate, and Golgi cells were also observed displaying a prominent cytosolic punctate labelling pattern, whereas Purkinje and granule cells displayed considerably less label. B, C: By contrast, ADC-like immunoreactivity was most prominent in cell bodies and branched dendritic trees (black arrow) of Purkinje cells and inhibitory interneurons in the molecular and granule cell layer. However, Bergmann glial cell bodies (white arrowhead) and processes (black arrowhead) and granule cells (white arrow) also were positive. With increasing antibody concentrations (compare 1∶5000 in B and 1∶1000 in C), the diffuse immunoreactivity in the neuropil became clearly evident. D: With SpdS labelling, cell bodies were hardly delineated. Instead, the neuropil of the molecular layer was strongly but diffusely immunopositive, while in the granule layer numerous giant mossy fiber-like boutons (arrowheads) were detected. E: Agm labelling was also markedly expressed in the molecular layer but most prominently observed in Purkinje cell bodies and denritic processes. Comparatively strong labelling was evident in the regionally occuring unipolar brush cells (arrows). ml = molecular layer, pl = Purkinje cell layer, gl = granule cell layer. Scale bar represents 50 µm.