RT-pPCR validation of microarray gene expression changes in human Met-5A pleural mesothelial cells following infection with D39 <i>Streptococcus pneumoniae</i>.

<p>Met-5A cells were infected with D39 (MOI 200 bacteria/cell) for 2h. RNA was extracted from pleural cells and analyzed on human microarrays. Mean fold expression changes were calculated for each gene from n = 5 experiments and a ≥ 2-fold increase/decrease was considered significant. RT-qPCR validation was done from n = 3 independent infection experiments in triplicate. The 10 most differentially up- and down-regulated genes are shown, as derived from IPA analysis, and the remainder can be seen in the gene datasets in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142773#pone.0142773.s005" target="_blank">S3A and S3B Table</a>. C, cytoplasm, Cy, cytosol; ES, extracelular space; PM, plasma membrane; N, nucleus; R, ribosomes.</p><p>* denotes statistical significance (Ct values) <0.05.</p><p>RT-pPCR validation of microarray gene expression changes in human Met-5A pleural mesothelial cells following infection with D39 <i>Streptococcus pneumoniae</i>.