SA-binding activity of HsGAPDH monitored by SPR analyses.

<p>(A-D) Sensorgrams of HsGAPDH flowing over 3AESA-immobilized CM5 sensor chip surface. (A) Concentration-dependent response to increasing amounts of HsGAPDH. (B) Concentration-dependent inhibition by SA of HsGAPDH (0.5 μM) binding to a 3AESA-immobilized sensor chip. (C) Suppression by amorfrutin derivatives B1, FN1, and FN2 of HsGAPDH (0.5 μM) binding to a 3AESA-immobilized sensor chip. (D) Suppression by 4-HBA, SA and 5-ASA of HsGAPDH (0.27 μM) binding to a 3AESA-immobilized sensor chip. The signal from the mock-immobilized surface was subtracted.