Real-time analysis of TNP-specific Ig-fLC binding to TNP-BSA coated SPR chips.

<p>Increasing concentrations of Ig-fLC (300 ng, 900 ng, 1.8 µg, 2.7 µg and 3.6 µg per CM5 sensor chip) resulted in increased binding curves (A). Administration of TNP-IgE (3.6 µg) resulted in significant binding to the TNP-coated surface and saturation around 350 s. Administration of equal amounts of TNP-Ig-fLC showed binding, yet no clear saturation was observed (B). Administration of TNP-BSA (1 µg/ml) completely eliminated the binding of Ig-fLC to the antigen-coated chip (C). No loss in binding was observed after non-conjugated BSA administration (data not shown). Results are representative for 3 individual experiments.