Analysis of the binding affinity of scFv-IC16 to immobilized Aβ peptides.

<p>(A: Aβ(1-8), B: Aβ(2-8) and C: Aβ(3-8)) using SPR. Depicted are the overlaid sensorgrams of different injection concentrations of scFv-IC16 onto an Aβ peptide coupled CM5 sensor chip. Each injection was performed for 3 min at a flow rate of 20 µl/min. Concentrations from 0 to 1000 nM were injected (see legend) in consecutive order. Dissociation was observed 2 to 4 min following an injection. Selected concentrations were injected twice as controls. The experimental data were fitted using the Langmuir 1∶1 binding model (Ri = 0). [25°C; Running buffer: PBS, RmaxAβ(1-8) = 67.5; RmaxAβ(2-8) = 553; RmaxAβ(3-8) = 126].