Single-cell transcriptome analysis of E13.5 female and male PGCs.

<p><b>(A)</b> Bi-dimensional <b>PCA</b> of E13.5 female and male single-PGC gene expression patterns. The first principal component (PC1) captures 4.93% of the gene expression variability and the second principal component (PC2) captures 4.25%. The red and blue spheres represent single female (n = 67) and male (n = 77) PGCs, respectively. <b>(B)</b> Hierarchical clustering analysis of transcriptome data sets (FSG: 651, MSG: 428) of female and male single PGCs. <b>(C)</b> Heat map of the relative expression of apoptotic process-related genes (n = 15). <b>(D)</b> Heat map of the relative expression of meiotic and mitotic cell cycle-related genes (n = 26). <b>(E)</b> Relation between apoptosis and meiotic and mitotic cell cycle in female PGCs. The numbers indicate the number of PGCs.