Human B-cell maturation into PC produces distinctive signatures.

<p><b>(A)</b> Principal Component Analysis (PCA) of the whole transcriptomes of BC, TPC, BPC and BMPC. <b>(B)</b> Heatmap of the average expression of differentially expressed probesets (adj. <i>P</i>-value < 0.1) in any pair-wise comparison. <b>(C)</b> PC-down signature. Left, Venn diagram showing the down-regulated genes in each PC compared to naïve BC: the central number represents the PC-down signature. Right, GO enrichment analysis of PC-down signature (<i>P</i>< 0.05, DAVID), in which the number of genes represents the mean ± SD of the genes contained in the GO terms within the indicated functional categories. <b>(D)</b> PC-up signature. Left, Venn diagram showing the up-regulated genes in each PC compared to naïve BC: the central number of the diagram represents the PC-up signature. Right, GO analysis as in (C) for the PC-up signature. <b>(E)</b> Box-and-whisker plots of the PC-down, BPC-up and PC-up genes that overlapped with the subset defined in GenomicScape as those genes with the highest deviation from average expression among naïve BC (NB), memory BC (MB) and BMPC from the resource [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0183264#pone.0183264.ref020" target="_blank">20</a>]. Numbers of overlapping genes are in brackets in the color legend. For clarity, outliers have been removed. *, <i>P</i> < 0.05 in Student’s t-tests between signatures within the same GenomicScape cell subtype; §, <i>P</i>< 0.05 Student’s t-tests between each cell subtype and NB within the same signature.