Effect of morin on MSU crystal-induced production of pro-inflammatory meditors in RAW 264.7 macrophage cells.

<p>(a) The levels of (i) PGE₂ and NO were measured in the culture medium. (b) The level of (i) TNF-α, (ii) IL-1β, (iii) IL-6, (iv) MCP-1, (v) VEGF and PGE₂ in the culture medium was measured by its respective ELISA kits. The results are expressed as means ±SD of the data from three experiments. Comparisons are made with: a—Control versus MSU crystal-induced RAW 264.7 macrophage cells, MSU+morin-treated RAW 264.7 macrophage cells (100–300 μM) and MSU+colchicine (1 μM) treated macrophage cells; b—MSU crystal-induced RAW 264.7 macrophage cells versus MSU+morin-treated RAW 264.7 macrophage cells (100–300 μM) and MSU+colchicine (1 μM) treated RAW 264.7 macrophage cells; c, d, e—MSU+morin treated RAW 264.7 macrophage cells (100–300 μM) versus MSU+colchicine (1μM) treated RAW 264.7 macrophage cells. *<i>P</i><0.05 statistically significant.