sEH gene disruption abolished sEH activities and sEH protein expression.

<p>Representative HPLC chromatograms showing the metabolism of 14,15-EET to 14,15-DHET by cytosolic fractions prepared from kidney and liver of WT (A and C) and sEH-KO (B and D) mice. The sEH activity in renal (C) and liver (F) cytosolic fractions from sEH-KO and WT mice revealed complete loss of activity by gene knock out. Data are given as mean ± SEM (n = 5–6 per group). Statistically significant differences were observed as indicated: ** p<0.01 vs WT. (G): Representative Western blot of liver and kidney homogenates from sEH-KO and WT mice.