Additional file 2: Figure S2. of Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate

SERS enhancement of different Raman reporters on spherical gold nanoparticles. Raman spectra were acquired for five different Raman reporter molecules; DTTC iodide, Crystal Violet, Malachite Green, 4-ATP, and 4-MBA. Sixty nanometer spherical gold nanoparticles were labeled with each reporter at a concentration of 400nM. After labeling, the Raman spectrum of each nanoparticle solution was acquired on our custom Raman microscope system. The intensity of the major peak in each spectrum was determined and compared. The Raman intensity of DTTC iodide is much greater than for other reporter molecules. (PDF 103 kb