Additional file 1: Figure S1. of Rational design of Raman-labeled nanoparticles for a dual-modality, light scattering immunoassay on a polystyrene substrate

Design of a custom Raman microscope system. The optical response of the SERS probes was detected using a custom Raman microscope system, which was constructed with an inverted microscope base, Figure S1A. With its inverted base and long working distance objectives lenses, the system was built to analyze SERS probes on a microplate substrate. The custom Raman system had a spectral resolution of 1.6 cm−1, a laser wavelength of 785nm, and a maximum power of 40mW. The Raman system captured spectral images using a 1340x400 pixel CCD detector. A Raman spectral image was obtained (Figure S1B, background image) and was averaged across each column to produce a Raman spectrum (Figure S1B, red overlay). The custom Raman system was used to acquire the Raman spectra of SERS probes in the development of light scattering immunoassays. (PDF 1673 kb