Internalization of CPP-conjugated anti-p24 mAbs into Jurkat and H9 T cells.

<p>Cells were co-cultured with CPP-anti-p24 mAbs at 37°C for 18 h. Cells were then treated with trypsin and washed with cold PBS before lysing. The cell lysates were centrifuged and clarified cell supernatants were used to determine CPP-anti-p24 mAb internalization by antibody capture ELISA as described by Zhou and co-workers [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0145986#pone.0145986.ref023" target="_blank">23</a>]. The results are representative of three independent experiments each done in duplicate. <b>(A)</b> CPP-anti-p24 mAb internalization into Jurkat T cells. <b>(B)</b> CPP-anti-p24 mAb internalization into H9 T cells.