The anti-Cdc37 antibody is cell impermeable.

<p>Anti-Cdc37 antibody internalization was tested in both MDA-MB 453 and MDA-MB 231 cells. Live cells from the two cell lines were grown on coverslips and incubated at 37°C for 16 h with anti-Cdc37. MAb 4C5 and anti-HSP90 were used as positive and negative controls respectively. For detection of antibody internalization, cells were fixed and permeabilized as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042722#s2" target="_blank">materials and methods</a> and antibodies were detected by confocal microscopy using an Alexa 488 secondary antibody. No internalization of the anti-Cdc37 antibody was observed even in both cell lines. Scale bar = 20 µm.