p24-producing cells from ART-suppressed individuals are enriched in T_TM/T_EM, PD-1+, TIGIT+ and α4+β1+ cells.

Representative dot plots showing the phenotype of p24+ cells represented as red dots overlaid onto all cells in grey. Purified CD4 T cells from 12 ART-suppressed individuals were stimulated with PMA/ionomycin for 24h before analysis. BFA was added 1h before PMA/ionomycin stimulation and was maintained until the end of the stimulation. The contribution of a given subset to the pool of all cells and to the pool of infected cells (p24+) are compared in samples from n = 12 ART-suppressed individuals (ART5, 9, 10, 11, 14, 16, 18, 19, 21, 22, 27, 33). (A) Immune checkpoint molecules: LAG-3, Tim-3, PD-1, TIGIT. (B) Memory phenotype: TN, TCM, TTM, TEM and TTD cells. (C) Integrins: α4β7 and α4β1. For statistical analyses, Wilcoxon matched-pairs signed rank test was performed.