Increased infiltration of CCR5-positive cells and activation of hepatic stellate cells in liver tissues of <i>Tet-mev-1</i> mice.

<p>Liver specimens were obtained from male wild type (<b>A</b>, <b>B</b>, <b>E</b> and <b>F</b>) or <i>Tet-mev-1</i> mice (<b>C</b>, <b>D</b>, <b>G</b> and <b>H</b>) that had been supplied with doxycycline-containing water and were subsequently fed control, normal chow (<b>A</b> to <b>D</b>) or a high-fat/high-sucrose diet (<b>E</b> to <b>H</b>) for 4 months at around the age of 2 years. The tissue sections were subjected to immunohistochemical staining using antibodies against CCR5 (<b>A</b>, <b>C</b>, <b>E</b> and <b>G</b>) or α-smooth muscle actin (<b>B</b>, <b>D</b>, <b>F</b> and <b>H</b>). CCR5-positive cells are shown by <i>arrows</i> in panels <b>E</b> and <b>G</b>, while α-smooth actin-expressing myofibroblasts were indicated by arrows in panel <b>H</b>. Representative images are shown from five mice in each group. <i>Scale bars</i>, 100 μm. A part of panels <b>E</b> to <b>H</b> is presented under high magnification in the corresponding panels on the right.