Detection of lipid peroxidation and apoptosis in liver tissues.

<p>Liver specimens were obtained from male wild type (<b>A, C, E,</b> and <b>G</b>) or <i>Tet-mev-1</i> mice (<b>B, D, F</b> and <b>H</b>) that had been supplied with doxycycline-containing water and were subsequently fed a control, normal diet (<b>A, B, E,</b> and <b>F</b>) or high-fat/high-sucrose diet (<b>C, D, G</b> and <b>H</b>) for 4 months at around the age of 2 years. They were subjected to 4-hydroxy-2-nonenal staining (<b>A</b> to <b>D</b>) or apoptosis detection using the TdT-mediated dUTP nick end labeling method (<b>E</b> to <b>H</b>). Hepatocytes and non-parenchymal cells positively stained for 4-hydroxy-2-nonenal are indicated by arrows and arrowheads, respectively, in panels <b>C</b> and <b>D</b>, while non-parenchymal cells showing apoptosis are shown by arrowheads in panels <b>F</b> and <b>H</b>. Representative images from five mice in each group are shown. <i>Scale bars</i>, 20 μm.