BMP signaling influences iGluR subtypes distribution.

<p>(A-D) Confocal images of NMJ4 boutons from third instar larvae of indicated genotypes labeled for GluRIIA (green), GluRIIB (red), and HRP (blue) (quantified in B-C). The relative intensity of postsynaptic GluRIIA and GluRIIB signals decreases unequally in mutants lacking synaptic pMad, and induces a reduction in IIA/IIB ratio, except for <i>gbb</i> mutants (A-B). Equal reduction of GluRIIA and GluRIIB signals (and thus normal IIA/IIB ratio) is found in larvae with Mav-depleted glia and Put-depleted muscle (C-D). Genotypes: control (<i>w</i>^<i>1118</i>), <i>gbb</i> (<i>gbb</i>^<i>1/Df</i>), <i>wit</i> (<i>wit</i>^{<i>A12/Df</i>}), <i>mad</i> (<i>mad</i>^<i>12/Df</i>), <i>imp</i> (<i>imp</i>^<i>24/70</i>), <i>G>mav</i>^<i>RNAi</i> <i>(repo-Gal4/UAS-mav</i>^<i>RNAi</i><i>)</i>, <i>M>put</i>^<i>RNAi</i> <i>(G14-Gal4/UAS-put</i>^<i>RNAi</i><i>)</i>. Error bars indicate SEM. ***; p<0.001, **; p<0.01, *; p<0.05. Scale bars: 5 μm and 1 μm (details).