Surface distribution of iGluR complexes at various Neto levels.

<p>(A) Confocal images of NMJ4 in third instar larvae of indicated genotypes labeled for GluRIIC (green), and HRP (red). Surface distribution of GluRIIC at synaptic and extrasynaptic locations was captured by immunohistochemistry with an N-terminal-specific antibody in detergent-free conditions. The GluRIIC signals were absent in the knockdown control (<i>G14>GluRIIC^RNAi</i>) and were shifted from synaptic to extrasynaptic locations at either low (<i>G14>neto^RNAi</i>) and excess Neto levels (<i>neto³⁶;G14>neto-A3</i> and <i>G14>neto-A1</i>) (quantified in B). The image insets are shown at higher intensity. The numbers of NMJs examined are indicated in each bar. Error bars indicate SEM. *; <i>p</i><0.001, **; p<0.01. Bars: 10 μm, 1 μm in details.