Activation of BMP signaling is suppressed in <i>Khc-73</i> mutant larvae.

<p>(A) pMad staining at the NMJ for Control (<i>BG380-GAL4</i>/+), Motoneuron>WIT (<i>BG380-GAL4</i>/+; <i>UAS-Wit</i>/+) and Motoneuron>Wit, <i>Khc-73</i> (<i>BG380-GAL4</i>/+; <i>UAS-Wit</i>, <i>Khc-73</i>^<i>149</i>/+, <i>Khc-73</i>^<i>149</i>). (B) Quantification of mean pMad fluorescence intensity for genotypes in (A). N = 22, 44 and 10 NMJs. (C) pMad staining at the NMJ for Control (<i>MHC-Gal4</i>/+), Muscle>Gbb (<i>UAS-Gbb</i>^<i>99</i>/+; <i>MHC-Gal4</i>/+) and Muscle>Gbb, <i>Khc-73</i> (<i>Khc-73</i>^<i>193</i>, <i>UAS-Gbb</i>^<i>99</i>/<i>Khc-73</i>^<i>149</i>, +; <i>MHC-Gal4</i>/+) (D) Quantification of mean pMad fluorescence intensity for genotypes in (C). N = 12, 12, and 15 NMJs. Error bars are S.E.M. Student’s t-test. *P<0.05, ***P<0.001. Scale bar is 10μm.