Serum antibodies in NOD mice with different treatments.

<p>Five-week-old female NOD mice were fed CTB-Ins-GAD (n = 6), CTB-GAD-Ins (n = 6), CTB-GAD (n = 6), or WT (n = 6) alone three to four times per week until 10 weeks of age. The serum antibodies were detected. (A) Anti-CTB, anti-insulin, anti-GAD65 IgG serum antibody titers; (B) anti-CTB IgG1, anti-insulin IgG1, and anti-insulin IgG2a; (C) anti-GAD65 IgG1, anti-GAD65 lgG2a; (D) anti-CTB IgA and IgE serum titers in mice fed CTB-Ins-GAD, CTB-GAD-Ins, CTB-GAD, or WT. Results are presented as the mean titer values ± SD. Six mice per group were individually tested in two separate experiments. *** P<0.001; NS: no significant difference.