Protein expression of first generation mutants.

SDS-PAGE showing expression of bifu25 and bifu29 and the other fusion proteins.

Shaista Arif (13883932)
Mohsina Akhter (13883935)
Aasia Khaliq (3906913)
Muhammad Waheed Akhtar (3131019)

September 2022

Lanes M: Protein markers; 1: Uninduced E. coli cells; 2: Lysate of the induced E. coli cells; 3: Sol...

SDS–PAGE analysis and gelatin gel zymography.

Sruti Dutta (409672)
Jiban Kanti Dattagupta (409673)
Sampa Biswas (409674)

May 2013

A. Purified and refolded pro-enzymes of mutants and wild-type were analyzed in 15% SDS-PAGE; M de...

Analysis of mutant protein expression. A. Surface expression of αIIbβ3 on HEK293 cells expressing normal or mutant αIIbβ3 receptors as judged by the binding of mAb 10E5.

Ming Cheng (494553)
Jihong Li (45207)
Ana Negri (60531)
Barry S. Coller (371201)

December 2013

1 million cells in 100 µl were incubated with 5 µg/ml Alexa488-conjugated mAb 10E5 (black line) o...

Protein expression.

Katia Pane (848426)
Lorenzo Durante (848427)
Elio Pizzo (730472)
Mario Varcamonti (26310)
Anna Zanfardino (848428)
Valeria Sgambati (848429)
Antimo Di Maro (654973)
Andrea Carpentieri (243434)
Viviana Izzo (730478)
Alberto Di Donato (730477)
Valeria Cafaro (848430)
Eugenio Notomista (730473)

January 2016

SDS-PAGE analysis of ONC-(P)GKY20 and ONC-DCless-H6-(P)GKY20 fusion proteins. Lane M1: marker (14...

SDS-PAGE analysis of the purified wild-type and mutant bglTm (T1 and T2) proteins.

Vitor M. Almeida (4768668)
Maira A. Frutuoso (4768671)
Sandro R. Marana (560928)

January 2018

Gels were stained with Coomassie Blue R. Arrows indicate the purified proteins. MW, molecular wei...

SDS-PAGE of protein expression and purification.

Yan-Feng Liu (326557)
Nan Zhang (46264)
Xi Liu (142824)
Xinquan Wang (211905)
Zhi-Xin Wang (156984)
Yuanyuan Chen (165812)
Hong-Wei Yao (326558)
Meng Ge (156979)
Xian-Ming Pan (40954)

February 2013

From left to right, every three consecutive lanes represent a protein, and the protein name is gi...

Fig 3 -

Satoshi Inouye (203922)
Jun-ichi Sato (133357)
Yuiko Sahara-Miura (1696846)
Yuri Tomabechi (10644646)
Yuto Sumida (1696852)
Shun-ichi Sekine (1372875)
Mikako Shirouzu (272392)
Takamitsu Hosoya (205623)

September 2022

SDS-PAGE analyses of the soluble fractions of the reverse mutants expressed in E. coli cells using a...

SDS-PAGE of the recombinant proteins.

Simei Fu (181750)
Jie Xu (34477)
Xianbo Li (181760)
Yongfei Xie (181768)
Yefeng Qiu (172131)
Xinying Du (181777)
Shuang Yu (181261)
Yaoxia Bai (181785)
Yanfen Chen (181787)
Tongkun Wang (181792)
Zhoujia Wang (181797)
Yaqing Yu (181802)
Guangneng Peng (181808)
Kehe Huang (172082)
Liuyu Huang (181816)
Yufei Wang (171069)
Zeliang Chen (181824)

February 2012

The expression of recombinant fusion proteins were analyzed by SDS-PAGE. M, molecular size protei...

SDS-PAGE analysis.

Er Meng (349309)
Tian-Fu Cai (349310)
Wen-Ying Li (349311)
Hui Zhang (7197)
Yan-Bo Liu (349312)
Kuan Peng (270181)
Songping Liang (349313)
Dong-Yi Zhang (308)

February 2013

A) Lane M, protein molecular weight markers; lane 1, uninduced cell sample; line 2, induced cell ...

MOESM4 of A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity

Natalie Rahmen (3496205)
Christian Schlupp (3496196)
Hitoshi Mitsunaga (3496214)
Alexander Fulton (2579620)
Tita Aryani (3496211)
Lara Esch (3496199)
Ulrich Schaffrath (3496208)
Eiichiro Fukuzaki (3496202)
Karl-Erich Jaeger (125594)
Jochen Büchs (3437060)

October 2015

Additional file 4. SDS-PAGE analysis for soluble and insoluble protein fractions of four clones pres...

SDS-PAGE of soluble and insoluble extracts (5 μl) of the best clones isolated after four rounds of mutagenesis, cloned in the pET23NN plasmid

Pascal Philibert (6237)
Pierre Martineau (6238)

December 2011

Copyright information:Taken from "Directed evolution of single-chain Fv for cytopla...

(A and B) Immunoblots demonstrate equivalent expression of GFP-MK and the indicated point mutant proteins

Manojkumar Valiyaveettil (90203)
Amber A. Bentley (90204)
Priya Gursahaney (90205)
Rajaa Hussien (69562)
Ritu Chakravarti (72891)
Nina Kureishy (90206)
Soren Prag (90207)
Josephine C. Adams (90208)

December 2011

Molecular mass markers are shown in kilodaltons. (C–F) Merged confocal images showing localization o...

Total (A) and soluble (B) expression for protein 1 – 30 (Table 1) with various N-terminal fusion partners analysed by SDS-PAGE fluorescence western blots as described in Materials and Methods

Michael R Dyson (42260)
S Paul Shadbolt (42272)
Karen J Vincent (42273)
Rajika L Perera (42270)
John McCafferty (42278)

December 2011

Copyright information:Taken from "Production of soluble mammalian proteins in Esche...

MAB21L2 wild-type and mutant protein studies.

Brett Deml (696234)
Ariana Kariminejad (696235)
Razieh H. R. Borujerdi (696236)
Sanaa Muheisen (349545)
Linda M. Reis (696237)
Elena V. Semina (334068)

February 2015

A. Western Blot analyses of MAB21L2 wild-type and mutant proteins (with N-terminal FLAG ta...

The reliability of tune translation level strategies and the SDS-PAGE analysis of protein expression.

Yang Song (1235364)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Long Liu (390523)
Guocheng Du (390526)
Jian Chen (15340)

June 2017

a: For 5’-gene sequence mutants, the biocatalyst activity was compared with minimal foldin...

SDS-PAGE showing expression of bifu25 and bifu29 and the other fusion proteins.

Shaista Arif (13883932)
Mohsina Akhter (13883935)
Aasia Khaliq (3906913)
Muhammad Waheed Akhtar (3131019)

September 2022

Lanes M: Protein markers; 1: Uninduced E. coli cells; 2: Lysate of the induced E. coli cells; 3: Sol...

SDS–PAGE analysis and gelatin gel zymography.

Sruti Dutta (409672)
Jiban Kanti Dattagupta (409673)
Sampa Biswas (409674)

May 2013

A. Purified and refolded pro-enzymes of mutants and wild-type were analyzed in 15% SDS-PAGE; M de...

Analysis of mutant protein expression. A. Surface expression of αIIbβ3 on HEK293 cells expressing normal or mutant αIIbβ3 receptors as judged by the binding of mAb 10E5.

Ming Cheng (494553)
Jihong Li (45207)
Ana Negri (60531)
Barry S. Coller (371201)

December 2013

1 million cells in 100 µl were incubated with 5 µg/ml Alexa488-conjugated mAb 10E5 (black line) o...

Protein expression.

Katia Pane (848426)
Lorenzo Durante (848427)
Elio Pizzo (730472)
Mario Varcamonti (26310)
Anna Zanfardino (848428)
Valeria Sgambati (848429)
Antimo Di Maro (654973)
Andrea Carpentieri (243434)
Viviana Izzo (730478)
Alberto Di Donato (730477)
Valeria Cafaro (848430)
Eugenio Notomista (730473)

January 2016

SDS-PAGE analysis of ONC-(P)GKY20 and ONC-DCless-H6-(P)GKY20 fusion proteins. Lane M1: marker (14...

SDS-PAGE analysis of the purified wild-type and mutant bglTm (T1 and T2) proteins.

Vitor M. Almeida (4768668)
Maira A. Frutuoso (4768671)
Sandro R. Marana (560928)

January 2018

Gels were stained with Coomassie Blue R. Arrows indicate the purified proteins. MW, molecular wei...

SDS-PAGE of protein expression and purification.

Yan-Feng Liu (326557)
Nan Zhang (46264)
Xi Liu (142824)
Xinquan Wang (211905)
Zhi-Xin Wang (156984)
Yuanyuan Chen (165812)
Hong-Wei Yao (326558)
Meng Ge (156979)
Xian-Ming Pan (40954)

February 2013

From left to right, every three consecutive lanes represent a protein, and the protein name is gi...

Fig 3 -

Satoshi Inouye (203922)
Jun-ichi Sato (133357)
Yuiko Sahara-Miura (1696846)
Yuri Tomabechi (10644646)
Yuto Sumida (1696852)
Shun-ichi Sekine (1372875)
Mikako Shirouzu (272392)
Takamitsu Hosoya (205623)

September 2022

SDS-PAGE analyses of the soluble fractions of the reverse mutants expressed in E. coli cells using a...

SDS-PAGE of the recombinant proteins.

Simei Fu (181750)
Jie Xu (34477)
Xianbo Li (181760)
Yongfei Xie (181768)
Yefeng Qiu (172131)
Xinying Du (181777)
Shuang Yu (181261)
Yaoxia Bai (181785)
Yanfen Chen (181787)
Tongkun Wang (181792)
Zhoujia Wang (181797)
Yaqing Yu (181802)
Guangneng Peng (181808)
Kehe Huang (172082)
Liuyu Huang (181816)
Yufei Wang (171069)
Zeliang Chen (181824)

February 2012

The expression of recombinant fusion proteins were analyzed by SDS-PAGE. M, molecular size protei...

SDS-PAGE analysis.

Er Meng (349309)
Tian-Fu Cai (349310)
Wen-Ying Li (349311)
Hui Zhang (7197)
Yan-Bo Liu (349312)
Kuan Peng (270181)
Songping Liang (349313)
Dong-Yi Zhang (308)

February 2013

A) Lane M, protein molecular weight markers; lane 1, uninduced cell sample; line 2, induced cell ...

MOESM4 of A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity

Natalie Rahmen (3496205)
Christian Schlupp (3496196)
Hitoshi Mitsunaga (3496214)
Alexander Fulton (2579620)
Tita Aryani (3496211)
Lara Esch (3496199)
Ulrich Schaffrath (3496208)
Eiichiro Fukuzaki (3496202)
Karl-Erich Jaeger (125594)
Jochen Büchs (3437060)

October 2015

Additional file 4. SDS-PAGE analysis for soluble and insoluble protein fractions of four clones pres...

SDS-PAGE of soluble and insoluble extracts (5 μl) of the best clones isolated after four rounds of mutagenesis, cloned in the pET23NN plasmid

Pascal Philibert (6237)
Pierre Martineau (6238)

December 2011

Copyright information:Taken from "Directed evolution of single-chain Fv for cytopla...

(A and B) Immunoblots demonstrate equivalent expression of GFP-MK and the indicated point mutant proteins

Manojkumar Valiyaveettil (90203)
Amber A. Bentley (90204)
Priya Gursahaney (90205)
Rajaa Hussien (69562)
Ritu Chakravarti (72891)
Nina Kureishy (90206)
Soren Prag (90207)
Josephine C. Adams (90208)

December 2011

Molecular mass markers are shown in kilodaltons. (C–F) Merged confocal images showing localization o...

Total (A) and soluble (B) expression for protein 1 – 30 (Table 1) with various N-terminal fusion partners analysed by SDS-PAGE fluorescence western blots as described in Materials and Methods

Michael R Dyson (42260)
S Paul Shadbolt (42272)
Karen J Vincent (42273)
Rajika L Perera (42270)
John McCafferty (42278)

December 2011

Copyright information:Taken from "Production of soluble mammalian proteins in Esche...

MAB21L2 wild-type and mutant protein studies.

Brett Deml (696234)
Ariana Kariminejad (696235)
Razieh H. R. Borujerdi (696236)
Sanaa Muheisen (349545)
Linda M. Reis (696237)
Elena V. Semina (334068)

February 2015

A. Western Blot analyses of MAB21L2 wild-type and mutant proteins (with N-terminal FLAG ta...

The reliability of tune translation level strategies and the SDS-PAGE analysis of protein expression.

Yang Song (1235364)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Long Liu (390523)
Guocheng Du (390526)
Jian Chen (15340)

June 2017

a: For 5’-gene sequence mutants, the biocatalyst activity was compared with minimal foldin...

SDS-PAGE showing expression of bifu25 and bifu29 and the other fusion proteins.

Shaista Arif (13883932)
Mohsina Akhter (13883935)
Aasia Khaliq (3906913)
Muhammad Waheed Akhtar (3131019)

September 2022

Lanes M: Protein markers; 1: Uninduced E. coli cells; 2: Lysate of the induced E. coli cells; 3: Sol...

SDS–PAGE analysis and gelatin gel zymography.

Sruti Dutta (409672)
Jiban Kanti Dattagupta (409673)
Sampa Biswas (409674)

May 2013

A. Purified and refolded pro-enzymes of mutants and wild-type were analyzed in 15% SDS-PAGE; M de...

Analysis of mutant protein expression. A. Surface expression of αIIbβ3 on HEK293 cells expressing normal or mutant αIIbβ3 receptors as judged by the binding of mAb 10E5.

Ming Cheng (494553)
Jihong Li (45207)
Ana Negri (60531)
Barry S. Coller (371201)

December 2013

1 million cells in 100 µl were incubated with 5 µg/ml Alexa488-conjugated mAb 10E5 (black line) o...

Protein expression of first generation mutants.

Abstract

Extracted data

Protein expression of first generation mutants.

Abstract

Extracted data

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