(A and B) Immunoblots demonstrate equivalent expression of GFP-MK and the indicated point mutant proteins

Molecular mass markers are shown in kilodaltons. (C–F) Merged confocal images showing localization of GFP-tagged muskelin point mutants (green) in COS-7 cells costained with DAPI (blue). Bars, 10 μm. (G) Quantification of subcellular localizations. Each column represents the mean from two to three independent experiments. Error bars indicate SEM. At least 500 cells were scored for each construct. The localization of each protein is also shown schematically.<p><b>Copyright information:</b></p><p>Taken from "Novel role of the muskelin–RanBP9 complex as a nucleocytoplasmic mediator of cell morphology regulation"</p><p></p><p>The Journal of Cell Biology 2008;182(4):727-739.</p><p>Published online 25 Aug 2008</p><p>PMCID:PMC2518711.</p><p>