Localization of RPS9-FLAG and RPS9-FLAG deletion mutants.

<p>A.) U2OS osteosarcoma cells were transfected with wt RPS9-FLAG and RPS9-FLAG deletion mutants as indicated in the figure and fixed 24 hours after transfection. Corresponding DAPI images are shown. Bar 10 µM. B.) Schematic representation of the different RPS9-FLAG chimeric proteins tested and their respective localization pattern. The patterns were defined as being cytoplasmic, predominantly nucleolar, nucleolar and cytoplasmic, even nuclear, throughout the entire cell, nucleoplasmic excluding nucleoli, or nucleoplasmic and cytoplasmic but excluding the nucleoli. Shown is the percentage of cells with a particular pattern based on 200 transfected cells analyzed for each construct.