P53 protein expression.

<p><b>A)</b> Representative Western blot of p53 and GAPDH expression in HUVEC after treatment for 1 or 6 hours with fresh media or iron (II) citrate. (Original images supplied.) <b>B)</b> Quantifications of GAPDH (median and interquartile range displayed). <b>C)</b> P53 expression relative to GAPDH, p value calculated by Dunn’s test post Kruskal Wallis. Note in all four experiments, p53/GAPDH increased at 1 hour (minimum 1.5 fold; mean 2.1 [95% confidence intervals 1.8, 2.4] fold), and returned to baseline by 6 hours. <b>D)</b> P53/GAPDH protein changes in HUVEC treated with 40μM iron (II) citrate. Box plots demonstrate median, interquartile range, and two standard deviations.