TLR4 knock out eliminate the effects of LPS on MSC proliferation and osteogenic differentiation.

<p><b>(A)</b> Wild type (WT) cells and TLR4 knock out (TLR4^-/-) cells were treated with 1000 ng/ml LPS for 6 days. Cell proliferation was examined by CCK-8 kit assay. <b>(B, C)</b> TLR4^-/- cells were treated with 1000 ng/ml LPS for 6 days. Cell proliferation was detected by EdU incorporation assay. <b>(B)</b> The statistical results. <b>(C)</b> Representative images of EdU staining (Scale bar: 50 μm). <b>(D, E)</b> MSCs were differentiated with 1000 ng/ml LPS for 7, 15, or 25 days. The osteogenic differentiation was detected by alizarin red staining and quantification. Graph <b>(D)</b> shows the representative images, and <b>(E)</b> is the quantification results. <b>(F)</b> ALP activity of TLR4^-/- MSC-differentiated cells in the presence of 1000 ng/ml LPS. Data are presented as mean ± SEM from four independent experiments. **<i>P</i><0.01.