Knockdown of TLR4 induces proliferation of HaCaT keratinocytes.

<p>(A) Growth of sh control and shTLR4 cells under puromycine selection visualized by light microscopy. (B) Knockdown of TLR4 induces cellular proliferation. The alteration of the proliferation was analyzed by BrDU proliferation assay. The significance was evaluated by t-test analysis on duplicates (n = 2) from three independent experiments (p*<0.05; p****<0.0001). As a control cells transfected with a control sh were used. (C) Expression of TLR4 on transcriptional level after TLR4 knockdown. The differential TLR4 expression in the control and shTLR4 cells was evaluated by qPCR using specific TLR4 primers. (D) Expression of TLR4 on protein level after TLR4 knockdown. The differential TLR4 expression was evaluated by conventional western blot analysis using a monoclonal anti TLR4 antibody (HTA 125).