Confocal images for HepG2.

<p>(a-d) untreated cells, (e-h) cells treated with Triton X-100 at 0.05% v/v, (i-l) cells treated with 16 μg/mL of (ACHC-β3hV-β3hK), and (m-p) cells treated with 16 μg/mL of coumarin-(ACHC-β3hV-β3hK). (a, e, i, m) show phase-contrast images, (b, f, j, n) show signal emissions for the 545 (green) and 405 (blue) nm filters, (c, g, k, o) show signals for the 640 (red) and 408 (blue) nm filters, and (d, h, l, p) show all signals. The oval encircles a zone where the β-peptide was detected, and co-localized with cell membrane, and the arrow points to the nucleus of the cell affected by the β-peptide.