Fluorescence microscopy images for compound 1 (5 µM concentration) in HepG2 cells.

<p>(A) 37°C treated cells for 3.75 hours and then fixed and imaged; (B) DAPI stained cells shown in (A); (C) live cells treated with <b>1</b> for 3.75 hours at 37°C and then imaged; (D) Cells were pre-cooled at 4°C for 30 min and then treated with <b>1</b> for 3.75 hours and then washed, fixed and imaged; (E) DAPI stained cells shown in (D); (F) Live cells treated with <b>1</b> for 3.75 hours at 4°C (as in D); (G) Cells pre-treated with 10 mM NaN₃ and 50 mM deoxy-D-glucose for 60 minutes and then with <b>1</b> for 3.75 hours and then washed, fixed and imaged; (H) DAPI stained cells shown in (G); (I) live cells treated with NaN₃ and deoxy-D-glucose (as in G).