Slow Fiber Mutant Mice Are Resistant to Diet-Induced Weight Gain.

<p>(A) Weekly weight change during high fat diet (HFD) feeding of wild-type (Wt) and miR-499 Tg female mice. (B and C) Inguinal (IWAT) fat pads were removed from Wt and miR-499 Tg female mice after 5 weeks of HFD. Representative explants of IWAT are shown in (B) with total weights of IWAT shown in (C). (D) Weekly weight change during HFD feeding of Wt and Sox6 mKO male mice. (E and F) Inguinal (IWAT) fat pads were removed from Wt and Sox6 mKO male mice after 8 weeks of HFD. Representative explants of IWAT are shown in (E) with total weights of IWAT shown in (F). (G and H) Percentage of BrdU-positive (BrdU+) adipocyte nuclei obtained from subcutaneous fat depots of Wt mice compared to female miR-499 Tg (G) and male Sox6 mKO mice (H). BrdU given during last week of HFD treatment in drinking water. Representative studies on P100-120 mice; n ≥ 8 per cohort, repeated ≥ 3 cohorts. Error bars indicate SEM. Statistical significance assessed by two-tailed Student’s t-test. *p<0.05, **p<0.01.