MPCR bias evaluated by the mixed plasmid samples.

<p>Thirty-three plasmids were mixed in the sample with three different pooling gradients (a, b, and c represent plasmid mixes 1, 2, and 3, respectively, details in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0152464#pone.0152464.s005" target="_blank">S3 Table</a>). Their replications are showed in d, e, and f. The observed frequencies of the thirty-three templates were calculated and compared with the expected pooling frequencies. The numbers in the top left corner (<b>b, c, e,</b> and <b>f</b>) represent the Pearson correlation coefficients between the observed and expected frequencies.